In this work, a straightforward technique for protein immobilization on Sylgard 184 is described. The method consists of a direct transfer of dried protein/salt solutions to the PDMS interface during the polymer curing. Such non-conventional treatment of proteins was found to have no major negative consequence on their integrity. The mechanisms of this direct immobilization were investigated using a lysine modified dextran molecule as a model. Clear experimental results suggested that both chemical bounding and molding effect were implicated. As a proof of concept study, three different proteins were immobilized on a single microarray (Arachis hypogaea lectin, rabbit IgG, and human IgG) and used as antigens for capture of chemiluminescent immunoassays. The proteins were shown to be easily recognized by their specific antibodies, giving antibody detection limits in the fmol range.

Introduction

The past decade has witnessed a fast expansion of micro fabricated devices and especially biochips and integrated microarrays. These developments, concomitant with the microfluidic and microdevice growth, have pushed technology developers to find new materials to overcome the main disadvantagesscost and technical requirement (clean room)s of glass and silicon, traditionally used for biochips fabrication.......

Langmuir, 23 (8), 4523 -4527, 2007. 10.1021/la070018o S0743-7463(07)00018-2
Web Release Date: March 14, 2007
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